ObjectivePluripotent cells have been identified in adipose tissue.Other groups have demonstrated in vivo differentiation of adipose tissue-derived mesenchymal stem cells(ADMSCs)into cells exhibiting biochemical and functional markers of cardiac myocytes,including spontaneous beating.Based on these observations, the aim of this research was to isolate ADMSCs from rat adipose tissue,and to study the effect of rehmannia glutinosa polysaccharides(RPS)on the biological characteristics of ADMSCs,and to observe the protective effects of RPS against adipose-derived mesenchymal stem cells(ADMSCs)injury induced by hydrogen peroxide(H_2O_2).Furthermore,detect the effect of RPS on the differentiation of ADMSCs into cardiacmyocytes.MethodsAccording to the aims mentioned above,the study was divided into there parts.First part Adipose tissue was obtained from male Wistar rat,and then treated with trypsinase and collagenase sequently.After centrifugated,ADMSCs was obtained,and then cultured in DMEM medium containing 10

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