Malignant tumor has become the main threat to human health. The metastasis to the distal organ is the leading causes of death, which based on the cell migration. PDGF and PDGFR mediated signal pathway is involved in the cell migration . Evidence shows that the over expression or the PDGE independent activation of PDGFR enhances the tumor cell metastasis by targeting the tumor cell directly or matrix indirectly like angiogenesis.Laboratory and clinical evidence shows that product of Java Brucea Fruit has the inhibitory effect on the tumor. However, little is known about the effective constituent of Java Brucea Fruit which plays its role and the possibly involved mechanisms. Also, the effect of Java Brucea Fruit on the cell migration is not clear. To elucidate the possible mechanisms through which the Java Brucea Fruit exert its inhibitory effect on tumor and especially the correlation between PDGFR and tumor.To elucidate the possible mechanisms through which the Java Brucea Fruit exert its inhibitory effect on tumor and especially the correlation between PDGFR and tumor, we stably transfect the PAE cells with the plasmid which contains the full length of PDGFR, RT-PCR is used to detect the expression level of PDGFRβ, then the exogenous PDGF is added to induce the wound repair, aiming to find that whether the PDGF i?s functionally responsive to PDGF; also the same model system is used to investigate that whether the Java Brucea Fruit extract has an inhibitory effect on the migration of PAEResult:One: positive transfectants which highly express PDGFRβby stable transfection of recombinant plasmid which contains the PDGFRβcDNA into the PAE cells and screen by G418. RT-PCR shows that the PAE cells highly express PDGFRβm?RNA.Two: wound healing shows that the exogenous PDGF promotes the repair of PAE and it is dose dependent. It verifies that the PAE cells highly express recombinant PDGFRβ,the PDGFRβhas been sucessfully transported and localized at the membrane, the PDGFRβbinds to the exogenous PDGF and transducer the signal to the target signal molecules to induce cell responses, suggesting that the model system of the migration mediated by PDGF signal pathway is beneficial to the screen the potential cell migration inhibitory drugs.Three: the analysis of the inhibition of cell migration by Java Brucea Fruit extract suggests that Java Brucea Fruit alcoholic extract has a strong inhibitory effect on the migration of PAE cells, showing a dose-dependent pattern. It is possible that the Java Brucea Fruit extract exert its the inhibitory effect on the tumor, partly through targeting the cell migration.Four: to find out the effective constituent which mainly mediates the effect of the Java Brucea Fruit alcoholic extract, we compare the inhibitory effect of Java Brucea Fruit alcoholic extracts in which the Java Brucea Fruit extracts is solubilized in different concentrations of alcohol. It shows that 70% alchol group shows the highest inhibitory effect, then50% alchol group and last the then25% alchol group, suggesting that the effective constituent is more likely to solubilize in the alcohol of lower polarity.Conclusions:we successfully establish the PDGFR mediated cell migration model and for the first time find that the Java Brucea Fruit extracts has a strong inhibitory effect on the cell migration. What’s more, we find that the effective constituent of Java Brucea Fruit extracts is more likely to solubilize in the alcohol of lower polarity

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